Module+Three+Review

[] [] This picture above shows Hershey and Chase (say that 5 times fast). I would like you to use the links above and your text and your workbook to get ready to talk about this experiment. Draw a diagram and explain the experimental procedure.

P32 ENTERS CELL AND USES CELL RNA TO DUPLICATE AND BECOME RADIO ACTIVE, OFTEN DESTRYOING CELLS DNA, THAN EXPLODING OUT OF CELL AND CONTAMINATING ANOTHER. (T2 EFFECTS E. COLI AND IS A BACTERIOPHAGE AND WORKS LIKE P32) S35 EFFECTS THE PROTEIN BUT BECAUSE DNA DOES NOT HAVE SULFUR IT IS NOT AFFECTED

What were the key results of the Hershey Chase Experiment? ? THAT DNA IS THE CARRIER OF GENETIC INFORMATION, NOT PROTEIN What did these results tell scientists about the nature of the genetic material? THAT IT IS THE DNA, IT IS THE ONE THAT REPRODUCES

Know the other scientists and their work leading up to Watson and Crick: Miescher Griffith Avery/McCarty Franklin 2. Replication Bubble. [] This is a picture of a bubble of replication. You should be able to draw something like this. Also be able to label and above (3’, 5’, lagging strand, leading strand, direction DNA polymerase reads, and helicase. Also, add where DNA Ligase would work and the origin of replication.

3. How did Watson and Crick figure out the exact configuration of the base pairing on the DNA double helix? Remember our discussion in class. 1. Hydrogen bonds between bases allows for easy unzipping, rezipping; 2. A=T, G=C according to Chargaff’s rules; 3. Purine must hook up with pyrimidine to keep the constant diameter of the double helix; 4. 2 bonds between A-T, 3 H-bonds between G-C.

I like the question in your workbook pg. 12: Give two reasons why in DNA replication, the nucleotides T always pairs with A and Cytosine always pairs with Guanine.

4. Describe 4 differences between the structures of a DNA and RNA molecule. (see page 5 in your workbook).

5. List the enzymes used in DNA replication and the enzymes used in Transcription/Translation. Tell the role of each enzyme and tell which direction they may proceed? Either direction or one way only.

6. Know the changes that a newly transcribed RNA molecule undergo before export from the nucleus as a fully functioning mRNA. (Cap, Tail, splicing) Draw the changes here and explain.

7. Please study your workbook. Read it over and over and try to make up some questions of your own. 8. Please know everything on the **Review Key Concepts Page** (24 of your workbook). Would you be able to fill this out from memory?

I may just Xerox a couple of pages of your workbook and have you fill them out to see that you have used your workbook and KNOW the workbook on the exam.

Practice drug problems: [] Don’t forget, this exam also has some questions from Chapters 2&3 (arrgh!!!) so don’t forget to review these.